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Dose-response : a publication of International Hormesis Society

S., Bergenstal, R

Standard reconstitution process documented in research literature: Allow the vial to reach room temperature before opening Use a sterile alcohol swab on both the peptide vial and the bacteriostatic water vial Draw the calculated volume of bacteriostatic water into a syringe Inject slowly along the side of the vial avoid injecting directly onto the peptide powder Gently swirl (do not shake) until fully dissolved The reconstituted solution should be clear and colorless Storage Guidelines Avoid repeated freeze-thaw cycles

Blood samples were obtained at baseline and during the fasting steady-state period (at 30, 20, 10 and 0 min during the last 30 min of the continuous tracer infusion) to assess plasma glucose and insulin concentrations, and isotopic enrichments of glucose and glycerol