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Smoking is addictive, and consequently, it can be a real challenge to quit
Although amplicon pyrosequencing has major advantages for human microbiome studies, it has also some limitations, such as the possible overestimation of OTU richness due to homopolymer errors (repeated nucleotides), inaccuracies of taxonomic identification due to the short length of the sequences and the introduction of primer and sequencing related biases 50,51,52
Some of the most common are: Its a well-known saying, but well worth reiterating: prevention is the best cure
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If you are coughing up blood as a smoker, do not ignore this sign
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