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The animals were housed in a light-controlled room with a 12-h light/dark cycle and were allowed access to food and water ad libitum

Subtypes of nicotinic acetylcholine receptors in nicotine reward, dependence, and withdrawal: evidence from genetically modified mice

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Young people use flavored nicotine products at a higher rate, and a study from 2022 found that higher nicotine levels in flavored e-cigarette products is tied to greater dependence and lower motivation to quit