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and PMN release large amounts of toxic oxygen free radicals and proteolytic enzymes, including elastase and collagenase, which act on lung elastin, mucin, basement membrane, and collagen fibers, leading to destruction of alveolar septa and interstitial fibrosis

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Cell pellets resuspended in BEGM + 0.5 PSA were filtered using a 70 m cell strainer, collected by centrifugation (5 min, 225 g , 4 C) and cryopreserved in freeze media (F-media, 30% FBS, 10% DMSO)