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The coverslips were then washed with PBS and the cells were fixed with 4% PFA for 15 min, washed with 0.05% Tween 20 (Promega, WI, USA) diluted in PBS (PBST), permeabilized with 0.2% Triton X-100 (Promega, WI, USA) for 10 min, and blocked with 3% BSA in PBST for 3 h

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"I don't know how a patient gets so lucky as to have three neonatal intensive care nurses onboard the same flight when she is in emergency labor, but that was the situation we were in," Glenn said

Health Articles Smoking is not only harmful to the smokers themselves but also to the non-smokers who are exposed to cigarette smoke