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Many people have already started by the time they are 18, Burgess said

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2.2 Determination of endogenous hormone in the tobacco plant and root Tobacco plant endogenous hormones, including IAA, JA, GA, and ABA, were measured using enzyme-linked immunoassay (ELISA) ( 2.3 Protein extraction, digestion, and desalting The protein extraction, digestion, and desalting steps followed the methods as described in previous publications ( 2.4 iTRAQ labeling and fractionation The peptides were dissolved in a 0.5-ml TEAB solution and then labeled according to the instructions provided by the iTRAQ-8 standard kit from SCIEX