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The functional roles of IRT1 (iron-regulated metal transporter), TTG1 ( transparent testa glabra ), RHL1 ( root hairless1 ), -tubulin, RML1 ( root meristemless1 ), and nematode resistance ( Mi ) genes, which are involved in root development, have been demonstrated by this vector ( Recently, VIGS has unraveled the functional redundancy among group III members in their requirements for plant development and plant immunity-associated ROS production (Zhou and Zeng, 2017)

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