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337 The MSU crystals are initially engulfed by macrophages, which then facilitate the assembly and activation of the NLRP3 inflammasome, preceded by priming through pathways that activate NF-B, such as those initiated by the engagement of Toll-like receptors (TLRs) within the TLR family

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Libraries were sequenced on a HiSeq 4000 device (Illumina, San Diego, CA) using a HiSeq 3000/4000 PE Cluster Kit and two 50-cycle SBS kits (Illumina, San Diego, CA), reaching an average mean depth of 61,000 (16% CV) and 50,000 (5% CV) mapped reads per cell per sample for the acute and sub-chronic models, respectively