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Whether youre storing tobacco for short-term use or long-term aging, maintaining 5565% humidity , keeping it away from heat and light , and checking it regularly will ensure it stays fresh, aromatic, and burns just right

The sections were then incubated with Alexa fluor 555-labeled secondary antibody (1:1000 in 1x PBS pH 7.4) (Invitrogen, Thermo Fisher Scientific) for 1 hour at room temperature in a dark, humidified chamber, followed by washing in PBS

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