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Chemical analysis To determine the nutrient contents in first flue-cured tobacco leaves, 1 kg leave sample was randomly taken in each treatment, in which the main veins were removed, and grinded and sieved through a 0.4 mm sieve before analyzing the chemical components ( N -methyl anabasine, -nicotyrine, anabasine, anatabine, isonicotenine and cotinine, were determined by gas chromatography-mass spectrometry method ( Soil organic C and total N were determined by the potassium dichromate volumetric method ( and NO) contents were measured by copperized cadmium reduction-diazotization coupling colorimetric method

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His journey ultimately points toward the necessity of living in the present and appreciating what he has, rather than perpetually seeking more

The films script is loosely based on the Raymond Chandler novel The Big Sleep