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We conducted an a priori power analysis using G*Power Software for independent samples Students t-test 22 , assuming the group difference in mean change of the score is 2 (standard deviation 1.3) 7 and aiming to detect a significance level of 1% and statistical power of 90%, a total sample size of 30 participants (15 in each group) was needed

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Though there are various types of substances people smoke, tobacco is considered the top priority due to its adverse side effects on human health

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The animal studies were approved by the Ethics Committee of Guangxi Medical University