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The best combination of the multiple inputs analyzed in this investigation, to achieve maximum actual and predicted transgenic plants, was at OD 600 = 0.8 for the LB4404 strain of Agrobacterium 300 mol/L acetosyringone 20 min immersion time

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Tobacco requires a lot of agrochemicals to ward off pests and diseases, which can also cause a number of side effects when breathed in all day long, or when coming into contact with skin