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Following solvent evaporation, the resulting residue was washed with 4 500 mL n -hexane, and then the remaining mass was further processed through open column chromatography (OCC) on silica gel 60 (0.0400.063 mm) (Merck KGaA, Darmstadt, Germany) using mixtures of CHCl 3 and MeOH (95:5, 90:10, 85:15, 80:20, 75:25, 70:30, 65:35, 60:40, 55:45, and 50:50) as mobile phase, yielding 8 fractions

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JG : I dont see how they can be because theres nothing about pipe tobacco that targets underage smokers