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The Sobs, Chao, ACE, Shannon, and Simpson indices of the moldy and healthy samples were compared

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In 1885, James Buchanan Duke acquired a license to use the first automated cigarette making machine (invented by James Albert Bonsack), and by 1890, Duke supplied 40 percent of the American cigarette market (then known as pre-rolled tobacco)

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Other viable in-vitro methods include the use of biobanks, biological samples often left over from clinical procedures, such as surgery, or from dead bodies