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In comparison, the annual mean PM 2.5 exposures increased by 2.35 g m 3 (95% CI, 2.112.59 g m 3 ) in Ohio Valley, 2.52 g m 3 (95% CI, 2.272.78 g m 3 ) in the Northeast, and 1.92 g m 3 (95% CI, 1.692.15 g m 3 ) in the Rocky Mountains

At day 4 post infection, cell supernatant containing the released virus was harvested, centrifuged at 500 g for 5 min, filtered through a 0.22 M sterile vacuum filtration system, aliquoted and stored at 80 C until further use