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This includes not only the planting, tending, and harvesting of tobacco leaf, but generally also includes initial processing steps, including curing tobacco to dry out the leaves and stems

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28 , 0.25 g of dried tobacco powder is taken, 25 mL of extract (acetic acid and ethanol volume fractions of 1% and 2%, respectively) is added, shaken for 1 h and the filtered supernatant is determined with a continuous flow analyser

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