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To induce differentiation of the NCI-H441 cell line in co-cultures, 1 M Dexamethasone was added in the ALI medium (complete M199)

J Exp Clin Med 3:252256 Chen L, Fan J, Chen H, Meng Z, Chen Z, Wang P, Liu L (2014) The IL-8/CXCR1 axis is associated with cancer stem cell-like properties and correlates with clinical prognosis in human pancreatic cancer cases

After being exposed to vape smoke however, the cells quickly became overwhelmed and unable to perform their bodily duties