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After deparaffinization, antigen retrieval and permeabilization, the samples were blocked and probed with the rabbit polyclonal antibodies against loricrin, involucrin (both Abcam, UK), filaggrin, KLK7 and TG1 (all three Invitrogen, USA) and appropriate anti-rabbit secondary antibody conjugated with Alexa Fluor 555 (Invitrogen, USA)
Control cooking smells if hunting from a campsite or deer camp
After flasks were shaken at 200 rpm for 1 hour, the extracts were filtered through a PTFE syringe filter (0.45 m, Merck KGaA, Darmstadt, Germany)
From a more down-to-earth point of view, this warm, enthusiastic finish makes itself felt in the wallet