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Differences in study population selections, definitions of early-onset smokers, and considerations for confounding variables may have also contributed to the inconsistent results from studies based on United States data 14,15,22,23,24
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The additive effects of nicotine on the severity of HFD-induced hepatic steatosis was associated with significantly greater oxidative stress, increased hepatic TG levels, higher incidence of hepatocellular apoptosis, inactivation (dephosphorylation) of AMPK, and activation of its downstream target ACC ( Figure 1 Indeed, these above studies, using various experimental models, demonstrated that nicotine further worsens HFD-induced hepatic steatosis
