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The frequency of micronucleated cells (MNC) was significantly elevated in exfoliated buccal epithelial cells from chewers of BQT and tobacco + lime,[] tobacco and areca nut mixture,[] regular users of masheri alone or masheri and BQT,[] people who used gudakhu for more than 5 years[] and in patients with oral cancer.[] A step-wise increase in MNC frequency was observed in samples from subjects with no tobacco habit, healthy SLT habitus, and those with oral leukoplakia.[] The MNC frequency in exfoliated oral mucosal cells from bidi rollers and tobacco processors who did not use tobacco in any form, but were exposed occupationally to bidi tobacco was significantly elevated as compared to controls with no tobacco exposure.[] MNC frequency was also elevated in cultured peripheral blood lymphocytes (PBL) from subjects who used masheri as a dentifrice.[] Cytogenetic alterations In a study that assessed toxic effects on allium , or onion bulbs treatment with tuibur was found to reduce root growth

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The Role of the Wrapper Leaf While the filler determines the structure and density of the ash, the wrapper leaf is responsible for its appearance

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If the case goes to court, they may issue at fine not exceeding $100