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Eosinophils (SiglecF + Ly6G - SSC High ) were sorted in PBS/1% FCS/5 mM EDTA using a BioRad S3 Cell Sorter (Bio-Rad)

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And on its confirmatory endpoint it found nothing: no significant separation from placebo, with only a non-prespecified hint of benefit in the most severely affected patients that the authors were careful not to oversell

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Peroxidated squalene induces the production of inflammatory mediators in HaCaT keratinocytes: a possible role in acne vulgaris J Investig Dermatol