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Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

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Blood Biomarkers of Intestinal Permeability Whilst the above methods represent direct measurement of molecules that are passing from the luminal side of the gastrointestinal epithelium to the basolateral side for measurement in blood or urine, there is also interest in finding suitable biomarkers for intestinal permeability and many have been proposed

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