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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

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Consensus sequence One approach that has been taken is to design vaccine antigens that are enriched for conserved T and B-cell epitopes either as a consensus sequence (which is the closest natural circulating sequence to a bioinformatically calculated consensus) or as a string of epitopes taken out of their natural context within the viral protein (Fig

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