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GLP-1 albumin fusions stimulate insulin secretion through a GLP-1R-dependent mechanism To test the ability of the GLP-1 albumin fusions to activate the GLP-1R in vitro, we used a human GLP-1R reporter cell assay kit, where serial dilutions of unfused GLP-1, GLP-1 WT, or GLP-1 QMP were incubated with the cells overnight (Fig

Role of covalent binding in vivo

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In line with this, the absorption of l-valine was also modest, supporting the theory that l-valine needs to be absorbed and metabolized to stimulate GLP-1 secretion

p values are shown in brackets