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Methods Animals All animal experiments were performed using male mice to ensure that sex difference influences were excluded, and all animals were housed in specific pathogen-free (SPF) facilities condition with 20-25 C temperature and 45-55% humidity on a regular 12-hour lightdark cycle, and approved by the Animal Welfare Ethics Committee at Renmin Hospital of Wuhan University (No

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ROS-induced oxidative damage is also responsible for mutations in mitochondrial DNA that inversely affect sperm motility by inhibiting energy production