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However, following weekly administration, B12 levels declined to a median of 530 pmol/L after 90 days

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This transition led to a decrease in the prevalence of infectious diseases and an increase in the majority of lifestyle-related diseases

Bacterial pellets were obtained by centrifugation for 15 min at 3,220 g and pellets were resuspended in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)