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doi: 10.1002/ptr.v34.9 46 SaradaSKTittoMHimadriPSaumyaSVijayalakshmiV

Determination of Michaelis-Menten constant for monobromobimane For validation of the assay, the enzymatic reaction between monobromobimane and glutathione was characterised to determine kinetic parameters such as apparent Michaelis-Menten constant and maximum rate of the reaction