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After their nucleotide sequences were confirmed, the coding regions of AsGGT1 , AsGGT2 , and AsGGT3 were cut out as Kpn I- Xho I fragments and were inserted between the Kpn I and Xho I sites in the yeast expression vector pYES2 (Life Technologies)
We used sulfonylureas as the active comparator as clinical guidelines used during the study period recommended DPP4 inhibitors and GLP-1-receptor agonists as well as sulfonylureas as second- or third-line glucose-lowering therapies [17], and no concern regarding an increased risk of cholangiocarcinoma with use of sulfonylureas has been raised
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