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Beaumont, R

a purification cycle was performed with condition of: Fractions containing liraglutide with 90.58% purity were adjusted with 33% Ammonium to pH value of 8.5 and diluted with 20 mM ammonium formate with pH value of 8.5, and then loaded onto RP-HPLC column packed with C8, 10 m silica having a diameter and length of 8 cm 12.6 cm

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Plated cells were pretreated with 4 L of agonist, or antagonist (Ex 9-39 (GLP-1R antagonist) or BIIE0246 (Y2-R antagonist)), at a given target concentration and incubated for 20 min before performing the assay