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Current markers (e.g., GPX4 expression, lipid peroxides, and serum creatinine) lack specificity for ferroptosis-driven disease, as they are altered in other glomerular diseases or metabolic disorders
Persistent severe symptoms warrant clinical review, as 3.9% of participants discontinued therapy due to GI intolerance, especially if fluid loss led to dehydration
Cells were grown at 37 C to an optical density at 600 nm (OD 600 ) of 0.70.8 in lysogeny broth (LPS solution LB-05) medium, and then 0.5 mM of isopropyl-1-thio--D-galactopyranoside (LPS solution IPTG025) was added
Meijer C, Mulder NH, Hospers GA, Uges DR, de Vries EG