Buford, L
Calibration standards were prepared by spiking 50 L of human plasma with appropriate amount of thiols to obtain the following concentrations: 20, 40, 60, 100, 200, 300, 600 nmol/mL plasma for Cys, 2, 4, 6, 10, 20, 30, 60 nmol/mL for Hcy, GSH and 0.1, 0.5, 1.0, 2.0, 4.0, 5.0, 10.0 nmol/mL for N-Hcy-Lys
The other half was fixed with 10% Formalin (Sigma) for 16 h at 4 C, transferred to a 70% v/v ethanol solution for a further 24 h and was then paraffin-embedded for immunohistochemistry
A key feature of this method is the use of a pretreatment column (50 mm 4.6 mm, i.d.) packed with poly(glycidyl methacrylate-divinylbenzene) microspheres, which facilitates the extraction and purification of analytes
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