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Determination of superoxide, hydrogen peroxide, root membrane injury and NADP(H)-oxidase Superoxide (O 2 ) content of the tissue was determined from plant tissue homogenized in 65 mM phosphate buffer (pH 7.8) and supernatant were collected by 8,000 g for 30 min, 4 C

doi: 10.1016/j.tox.2024.153958 61 PalombaSPiltonenTTGiudiceLC

Sun Y, Bak B, Schoenmakers N, van Trotsenburg AS, Oostdijk W, Voshol P, Cambridge E, White JK, le Tissier P, Gharavy SN, Martinez-Barbera JP, Stokvis-Brantsma WH, Vulsma T, Kempers MJ, Persani L, Campi I, Bonomi M, Beck-Peccoz P, Zhu H, Davis TM, Hokken-Koelega AC, Del Blanco DG, Rangasami JJ, Ruivenkamp CA, Laros JF, Kriek M, Kant SG, Bosch CA, Biermasz NR, Appelman-Dijkstra NM, Corssmit EP, Hovens GC, Pereira AM, den Dunnen JT, Wade MG, Breuning MH, Hennekam RC, Chatterjee K, Dattani MT, Wit JM, Bernard DJ

Take them for six months, lose weight, then stop and maintain through lifestyle changes alone

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