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325 A separate study examining 40 patients with Parkinsons disease receiving 90 mg/day (titrated over the course of 11 weeks) of transdermal nicotine for 28 weeks found no significant difference in UPDRS motor scores between treated and non-treated groups after six weeks of washout

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

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doi: 10.1016/j.clineuro.2016.01.029 133 KaravitakiNDobrescuRByrneJVGrossmanABWassJAH