A 10-parameter panel, including not only classical antioxidant tests (FRAC, Trolox, DPPH) but also endogenous antioxidant enzymes and protein degradation, has shown its advantages in stratifying OS status in different HTN degrees (Nuez-Sells et al., 2019)
Using ImageJ software, we quantified the number of cells positive for BrdU, TUNEL or ATF4 relative to the total number of chondrocytes in the different subregions of interest or in the total growth plate
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This process involved metabolic dysfunction that results in increased production of cytosolic and lipid ROS, independently of mitochondria