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RESULTS Liver lesions and biochemistry assessment In controls, the grading system used to microscopically measure the extent of liver damage (the same for steatosis, necrosis, and congestion) showed no lesion presentation at the earliest point of 25 minutes after paracetamol ( Table 1 ), but liver enzymes and ammonium serum values were already increased ( Table 2 )

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Step 2: Add 100ul standard/sample/biotin-labeled antigen working solution into each well