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For specific analysis of cytoplasmic and nuclear proteins, the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit was employed for TM3 cells

[21] Within cells, cyanocobalamin is converted to methylcobalamin and 5- deoxyadenosylcobalamin, coenzymes that participate respectively in homocysteine remethylation to methionine and in the isomerization of methylmalonyl-CoA to succinyl CoA, reactions essential for DNA synthesis, erythropoiesis, and myelin maintenance

In other words, it prevents your formulas from separating into an oily mess

Preconditioning limits mitochondrial Ca(2+) during ischemia in rat hearts: Role of K(ATP) channels