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Briefly, biopsies were flushed with 10 ml of ice cold PBS and minced into the small pieces

As a result, whether the observed alterations in the colchicine-induced model align with the inflammatory hypothesis of AD remains an open question

Indian J Physiol Pharmacol

In both scenarios the key is consistent administration timing, tracking outcomes (sleep quality, restorative rest, stress resilience), and maintaining product/formulation integrity