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Maisch, Germany, Ammerbuch-Entringen) analytical column (prepared in-house) at a constant pressure of 925 bar (flow rate of circa 700 nL/min) with 1% formic acid in water and eluted at a flow of 0.5 L min 1 with a 50 min linear gradient from 9% to 34% acetonitrile in water and 1% formic acid with a Thermo Vanquish Neo nanoLC (Thermo Fisher, Waltham, MA)

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Plant Mol Biol Mar 105(45):365383

Melville DB