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They were acclimatized in well aerated cages with alternating 12 hour light and dark cycles, maintained on standard rat chow and allowed free access to drinking water according to the regulations and ethics governing the use of animals in laboratory experiments of the University of Ibadan

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Zinman B et al

Beyond the protein-based methodologies used to characterise glicentin, GRPP and oxyntomodulin, the increased capability to sequence genes at the time led to the first sequence of mammalian preproglucagon (15)

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