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Next, the membranes were incubated overnight at 4 C with a specific primary antibody, followed by secondary antibody incubation at room temperature for 1 h

Maybe the spark is gone

Acute elevation of ICP needs timely treatment, so it's important to look for some warning signs such as headache, altered sensory, papilledema, hypertension, bradycardia or tachycardia, signs of herniation, retinal hemorrhages, bluish skin lesions and fractures

It can also lead to more serious problems, such as heart attacks and strokes

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