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73 Mitochondria, once again, become fragile targets in the pathogenesis of OA

2.18 RNA sequencing and transcriptome analysis 2.18.1 RNA extraction and library preparation Total RNA was extracted from cells using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturers instructions

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The ratio of GSH:GSSG (glutathione redox ratio) is a sensitive index of oxidative stress, which can lead to a toxic imbalance between the production and removal of reactive oxygen species (ROS)