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After deparaffinization, rehydrating, antigen retrieval, quenching and blocking, slides were incubated with primary antibody (-SMA, GFP (HuaBio, Cat# ET1602-7)) at 4 C overnight, applied with peroxidase conjugated secondary antibody in the diluent for 1 h at room temperature, stained with 3, 3-diaminobenzidine reaction solution, and imaged by Olympus digital slicing scanner VS200

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Additionally, alterations in GGT, BUN, ALP, lipase, and amylase levels reflect stress on hepatic and renal systems, as well as the pancreas [51, 52]

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