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CHIR99021 was removed after 72 h and DE cells were cultured in RPMI/B27 + Insulin for 24 h ( For growth factor Definitive Endoderm (DE) differentiation, human iPSCs cultured in 6-well dishes to 60% confluence were washed sequentially with DPBS / and DMEM/FI2 medium (Thermo Fisher Scientific)

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Murine models were inoculated with murine B16 melanoma (B16M) to obtain in vivo metastatic melanoma that was subsequently tested with the above-stated combination treatments

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For instance, if your doctor has prescribed only 3 cc in one dose, you may need to select a small syringe that holds the medicine properly