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Biotin, however, can interfere with your thyroid test results. According to the American Thyroid Association (ATA), "biotin use can result in falsely high levels of T4 and T3 and falsely low levels of TSH, leading to either a wrong diagnosis of hyperthyroidism or that the thyroid hormone dose is too high." Many multivitamins contain from 30 to 300 mcg of biotin, and supplements designated explicitly for hair, skin, and nails can include 5,000 to 10,000 mcg of biotin