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Some polyphenols, like curcumin and crocin, also enhance serotonin biosynthesis by upregulating TPH, the key enzyme in serotonin production [209, 210]

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Fixed cells were washed twice in PBS containing 10% serum and stained with 50 g ml 1 propidium iodide solution containing 100 g ml 1 RNase A for 1 h at 37 C

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This drying time is what actually kills the germs and ensures the site is properly disinfected