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Managing stress through structured physical activity, consistent routines, and intentional recovery is not a soft lifestyle recommendation

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10 l reaction mix per sample was as follows: 5 l of iTaq universal SYBR Green reaction mix (2x), 0.125 l of iScript reverse transcriptase (RT), 0.3 l of forward and reverse primers each (300 nM each), 2 l of template RNA (20 ng per reaction) and 2.275 l of nuclease free H 2 O

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