determination of glutathione The optimised method HPLC analysis of allows to determine the degree of oxidative stress in plant cell culture Glutathione-capped CuO nanoparticles for the

F.G.: AstraZeneca, Boehringer-Ingelheim, Eli Lilly, Lifescan, Medimmune, Merck Sharp & Dohme, Medtronic, Novo Nordisk, Roche Diabetes Care, Sanofi Aventis

In a microwell ELISA plate pre-coated with anti-mouse glutathione peroxidase (GSH-Px) antibody (solid phase antibody), add mouse glutathione peroxidase (GSH-Px) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid surface of the microwell plate

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Immunoblot signals were developed using EzWestLumi plus (ATTO) and recorded using an ImageQuant LAS 4000mini image analyzer (GE Healthcare, Japan)

Kautzky-Willer, A., Harreiter, J