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13 It is estimated that the economic burden of COPD in the next 20 years will increase by $40 billion per year

Ventilation strategy using low tidal volumes, recruitment maneuvers, and high positive end-expiratory pressure for acute lung injury and acute respiratory distress syndrome: a randomized controlled trial

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Pharmacological studies have demonstrated that these TCM-derived compounds exhibited therapeutic potential against AP by suppressing inflammatory response (Table 2

After washing steps, DNA was eluted twice with elution buffer (25 mM TrisHCl pH 7.5, 5 mM EDTA pH 8.0, 0.5% SDS, 65 C, 20 min), treated with 10 mg/mL RNase-A (65 C, 1 h) and with 20 mg/mL proteinase K (65 C, 5 h), then was purified using the AxyPrep DNA Purification Kit (Axygen, AP-GX-250)