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Subsequently, we conducted another review using the keywords "oxidative stress" and "infertility" in specialized literature and confirmed a close relationship between oxidative stress and infertility not only in fish but also in mammals and birds

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Cell culture studies have shown that NNMT inhibition increases intracellular NAD+ levels, enhances sirtuin activity, and shifts adipocyte metabolism toward greater fatty acid oxidation

Short culture period

Each experiment was conducted independently three times with duplicate measurements to ensure reproducibility and statistical robustness [1]