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Moreover, AMPK can enhance Sirtuin-1, the NAD + -dependent deacetylases family member owning deacetylating activity (Wang et al., 2019), through increasing cellular nicotinamide adenine dinucleotide (NAD + ) levels

First, the researchers developed an in vitro model simulating DED by exposing HCECs to hydrogen peroxide (H 2 O 2 ) and a hypertonic solution (HS) to mimic a dry eye environment